• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-SPOP

SPOP Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

N° de cat : 16750-1-AP

Synonymes

HIB homolog 1, Roadkill homolog 1, speckle type POZ protein, SPOP, TEF2



Applications testées

Résultats positifs en WBcellules HeLa, cellules HepG2, cellules PC-3
Résultats positifs en IF/ICCcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

16750-1-AP cible SPOP dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène SPOP Protéine recombinante Ag10215
Nom complet speckle-type POZ protein
Masse moléculaire calculée 374 aa, 42 kDa
Poids moléculaire observé 42 kDa
Numéro d’acquisition GenBankBC003385
Symbole du gène SPOP
Identification du gène (NCBI) 8405
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The SPOP (TEF2) protein was previously identified as an autoantigen in a patient with scleroderma pigmentosum. SPOP (speckle-type POZ protein), also known as TEF2, HIB homolog 1 or Roadkill homolog 1, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF Homology) domain and one C-terminal BTB/POZ domain. SPOP can exist as a homodimer and is expressed in a variety of tissues localizing to the nucleus. BTB-mediated SPOP dimers form linear oligomers via BACK domain dimerization, and we determine the concentration-dependent populations of the resulting oligomeric species (PMID: 27220849 ). Through an interaction with CUL-3, SPOP is involved in ubiquitinylation and protein degradation. SPOP specifically interacts with CUL-3 via its BTB/POZ domain and recruits substrates to the CUL-3-based ubiquitin ligase via its MATH domain. Substrates recruited by SPOP and targeted for ubiquitylation via the CUL-3/SPOP complex include PDX-1, Bmi-1, MacroH2A, PIPK II ∫ and Daxx. These substrates are subsequently degraded by the proteasome. In addition, SPOP itself becomes ubiquitylated by the CUL-3-based ubiquitin ligase and is targeted for proteasomal degradation.

Protocole

Product Specific Protocols
WB protocol for SPOP antibody 16750-1-APDownload protocol
IF protocol for SPOP antibody 16750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nature

Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance.

Authors - Jinfang Zhang
  • KO Validated
humanWB

Nat Med

Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.

Authors - Xiangpeng Dai
  • KO Validated
humanWB

Nat Med

Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.

Authors - Pingzhao Zhang
  • KD Validated
humanWB

Cell Res

ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer.

Authors - Kai Li
  • KD Validated
mouse

Mol Cell

DNA Damage Promotes TMPRSS2-ERG Oncoprotein Destruction and Prostate Cancer Suppression via Signaling Converged by GSK3β and WEE1.

Authors - Zhe Hong
humanWB

Mol Cancer

SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.

Authors - Qiwei Jiang
  • KD Validated