Anticorps Polyclonal de lapin anti-PBK

• Phare
• Validé par KD/KO

PBK Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

9

N° de cat : 16110-1-AP

Synonymes

MAPKK-like protein kinase, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2, CT84

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Galerie de données de validation

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  WB analysis using 16110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16110-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16110-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16110-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16110-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 10x lens).

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  FC experiment of HepG2 using 16110-1-AP

  (blue)1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PBK (16110-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu ovarien de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer de la peau humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

16110-1-AP cible PBK dans les applications de WB, IHC, FC (Intra), IP, CoIP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PBK Protéine recombinante Ag9033
• Nom complet: PDZ binding kinase
• Masse moléculaire calculée: 322 aa, 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC015191
• Symbole du gène: PBK
• Identification du gène (NCBI): 55872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).

Publications

Species	Application	Title
human	WB	Genomics Proteomics Bioinformatics; Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.; Authors - Yixian Cun
mouse	WB,IF	FASEB J; Autophagy plays a positive role in induction of epidermal proliferation.; Authors - Junyi Wang
mouse	IF	Invest Ophthalmol Vis Sci; Single-Cell Transcriptomics Reveals Cellular Heterogeneity and Complex Cell-Cell Communication Networks in the Mouse Cornea; Authors - Yueh-Feng Wu
human	IHC	Biomolecules; Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods; Authors - Haofuzi Zhang
mouse	WB,IF	Invest Ophthalmol Vis Sci; Single-Cell RNA Transcriptome Helps Define the Limbal/Corneal Epithelial Stem/Early Transit Amplifying Cells and How Autophagy Affects This Population.; Authors - Nihal Kaplan ; KD Validated
rat	WB	Front Endocrinol (Lausanne); PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas.; Authors - Kejing Zhu