Anticorps Polyclonal de lapin anti-Spartin, SPG20

• Phare
• Validé par KD/KO

Spartin, SPG20 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 13791-1-AP

Synonymes

KIAA0610, SPARTIN, Spartin, SPG20, Spastic paraplegia 20 protein, SPG20, TAHCCP1

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Galerie de données de validation

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  WB analysis using 13791-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13791-1-AP

  WB result of Spartin, SPG20 antibody (13791-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Spartin, SPG20 transfected HeLa cells.

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  IP experiment of HeLa using 13791-1-AP

  IP result of anti-Spartin, SPG20(IP:13791-1-AP, 4ug; Detection:13791-1-AP 1:2000) with HeLa cells lysate 2320 ug.

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  IHC staining of human liver cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13791-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 13791-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Spartin, SPG20 (13791-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

13791-1-AP cible Spartin, SPG20 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Spartin, SPG20 Protéine recombinante Ag4815
• Nom complet: spastic paraplegia 20 (Troyer syndrome)
• Masse moléculaire calculée: 75 kDa, 85 kDa
• Poids moléculaire observé: 75-84 kDa
• Numéro d’acquisition GenBank: BC047083
• Symbole du gène: SPG20
• Identification du gène (NCBI): 23111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.

Publications

Species	Application	Title
human	WB	Ann Neurol; Developmental and degenerative features in a complicated spastic paraplegia.; Authors - Manzini M Chiara MC
human	WB	Elife; Chromosome mis-segregation and cytokinesis failure in trisomic human cells.; Authors - Joshua M Nicholson
mouse	WB,IF	Hum Mol Genet; Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.; Authors - Renvoisé Beno?t B ; KO Validated
human	WB,IF	Oncogene; SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.; Authors - Lind G E GE
mouse	WB	J Cell Sci; Germline Deletion of Huntingtin Causes Male Infertility and Arrested Spermiogenesis in Mice.; Authors - Jinting Yan
human	WB,IF	FASEB J; A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism.; Authors - Chiara Diquigiovanni ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

beyenech (Verified Customer) (11-22-2019)

The antibody works also for Immunoprecipitation

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: IF: 1:100; WB:1:1000
• Cell Tissue Type: 293T and HeLa

Spartin, SPG20 Antibody Western Blot,Immunofluorescence, validation (IF: 1:100; WB:1:1000 dilution) in 293T and HeLa (Cat no:13791-1-AP)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C