Anticorps Polyclonal de lapin anti-SPERT

• Phare
• Validé par KD/KO

SPERT Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17826-1-AP

Synonymes

CBY2, NURIT, Protein chibby homolog 2, spermatid associated, Spermatid associated protein, SPERT

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Galerie de données de validation

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  WB analysis of mouse testis using 17826-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 17826-1-AP

  WB result of SPERT antibody (17826-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPERT transfected PC-3 cells.

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  WB analysis of PC-3 using 17826-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spermatophore using 17826-1-AP

  mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 17826-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SPERT antibody (17826-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules PC-3, tissu de spermatophore de souris
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17826-1-AP cible SPERT dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPERT Protéine recombinante Ag12200
• Nom complet: spermatid associated
• Masse moléculaire calculée: 448 aa, 47 kDa, 52 kDa
• Poids moléculaire observé: 48-52 kDa
• Numéro d’acquisition GenBank: BC027609
• Symbole du gène: SPERT
• Identification du gène (NCBI): 220082
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]

Publications

Species	Application	Title
human,mouse	WB,IF	Reprod Biol; Identification of a novel mutation in chibby family member 2 in a non-obstructive azoospermic patient; Authors - Guohui Zhang