Anticorps Polyclonal de lapin anti-SPDEF

SPDEF Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11467-1-AP

Synonymes

PDEF, Prostate derived Ets factor, Prostate specific Ets, PSE, RP11 375E1__A.3, SPDEF

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11467-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11467-1-AP cible SPDEF dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPDEF Protéine recombinante Ag2020
• Nom complet: SAM pointed domain containing ets transcription factor
• Masse moléculaire calculée: 335 aa, 38 kDa
• Poids moléculaire observé: 38-44 kDa
• Numéro d’acquisition GenBank: BC021299
• Symbole du gène: SPDEF
• Identification du gène (NCBI): 25803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell; A modular master regulator landscape controls cancer transcriptional identity.; Authors - Evan O Paull
human	WB,IHC	Clin Sci (Lond); Gut microbiota-derived butyrate regulates gut mucus barrier repair by activating the macrophage/WNT/ERK signaling pathway.; Authors - Liping Liang
human	WB,IF	Phytomedicine; Resveratrol inhibits MUC5AC expression by regulating SPDEF in lung cancer cells.; Authors - Yu-Hua Lin
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang
mouse	WB	Immunobiology; Lactobacillus rhamnosus 76 alleviates airway inflammation in ovalbumin-allergic mice and improves mucus secretion by down-regulating STAT6/SPDEF pathway; Authors - Yangfan Hou
rat	WB	Dig Dis Sci; Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats.; Authors - Linhao Zhang