Anticorps Polyclonal de lapin anti-SPCS2

• Phare
• Validé par KD/KO

SPCS2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 14872-1-AP

Synonymes

KIAA0102, SPase 25 kDa subunit, SPC25, SPCS2

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Galerie de données de validation

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  WB analysis using 14872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14872-1-AP (SPCS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 14872-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14872-1-AP (SPCS2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 14872-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14872-1-AP (SPCS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse colon using 14872-1-AP

  IP result of anti-SPCS2 (IP:14872-1-AP, 4ug; Detection:14872-1-AP 1:500) with mouse colon tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu de côlon de rat, tissu de côlon de souris, tissu de côlon humain, tissu d'estomac de souris, tissu splénique de souris
• Résultats positifs en IP: tissu de côlon de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

14872-1-AP cible SPCS2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPCS2 Protéine recombinante Ag6640
• Nom complet: signal peptidase complex subunit 2 homolog (S. cerevisiae)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC064957
• Symbole du gène: SPCS2
• Identification du gène (NCBI): 9789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal peptidase complex subunit 2 (SPCS2) is multi-pass membrane protein as a component of the microsomal signal peptidase complex, which function to remove signal peptides from nascent proteins before translocation into the endoplasmic reticulum lumen. This protein was evidenced as a 25 kDa protein with function not well known.

Publications

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang ; KO Validated
human	WB	EMBO J; Intramembrane client recognition potentiates the chaperone functions of calnexin; Authors - Nicolas Bloemeke

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Matthias (Verified Customer) (10-09-2019)

Strong band at ca. 20 kDa; fainter, most likely non specific, at ca. 25 kDa; some background, but overall decent antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

SPCS2 Antibody Western Blot, validation (1:1000 dilution) in 293T (Cat no:14872-1-AP)