• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-SPARC

SPARC Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 15274-1-AP

Synonymes

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC



Applications testées

Résultats positifs en WBcellules A375, cellules ROS1728, tissu placentaire humain, tissu testiculaire humain
Résultats positifs en IHCtissu de cancer de l'estomac humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochimie (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15274-1-AP cible SPARC dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéerat, Humain, souris, cerf Sika
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène SPARC Protéine recombinante Ag7390
Nom complet secreted protein, acidic, cysteine-rich (osteonectin)
Masse moléculaire calculée 35 kDa
Poids moléculaire observé35-43 kDa
Numéro d’acquisition GenBankBC004974
Symbole du gène SPARC
Identification du gène (NCBI) 6678
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Secreted Protein Acidic And Cysteine Rich (SPARC), also known as osteonectin or basement-membrane protein 40 (BM-40), is a secreted protein essential in the calcification of bone. 

What is the molecular weight of SPARC?

The calculated molecular mass of SPARC is 35 kDa. SPARC is a glycoprotein consisting of 303 amino acids in the cytoplasm and 286 amino acids after the signal sequence is cleaved in secretion. 

Where is SPARC expressed?

Osteoblasts secrete SPARC during bone formation, with high levels found in immature bone compared to mature bone that is in homeostasis (PMID: 2440898). SPARC is also secreted in adult mineralized tissues that have high turnover, such as osteoid and dentin, by cells other than osteoblasts. This includes bone marrow progenitor cells and hypertrophic chondrocytes but also endothelial cells and fibroblasts.

Though previously described as being exclusively expressed in mineralized tissue, it is now understood that SPARC is more widely expressed and is associated with high levels of collagen deposition, found in the extracellular matrix (ECM) of a number of tissues.

What is the function of SPARC?

SPARC contains both a collagen-binding domain and a hydroxyapatite (HA) binding region, so is thought to enhance mineralization by binding both collagen and HA crystals, causing the release of calcium ions (PMID: 26851678).

SPARC can bind to a number of different ECM proteins, particularly collagen, and may even influence the assembly of these proteins (PMID: 19798598). It is known to regulate interactions between cells and the ECM, meaning it plays a role in many important processes such as cell migration and proliferation.

What diseases are associated with SPARC?

Overexpression of SPARC has been identified in a number of different types of cancers (PMID: 18849185), although its role may vary. For example, in neuroblastomas SPARC acts as a suppressor but in gliomas, it causes greater invasion of the tumor. Mutations in SPARC can also lead to Osteogenesis Imperfecta, also known as brittle bone disease (PMID: 26027498).


Protocole

Product Specific Protocols
WB protocol for SPARC antibody 15274-1-APDownload protocol
IHC protocol for SPARC antibody 15274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Appl Mater Interfaces

Evidence for Delivery of Abraxane via a Denatured-Albumin Transport System.

Authors - Maichi Hama
humanIHC

Cancer Discov

PMID: 22585996

Authors - Frese Kristopher K KK
humanWB,IF

Theranostics

A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.

Authors - Lindsay B Alcaraz
ratIHC

Clin Cancer Res

The pancreas cancer microenvironment.

Authors - Feig Christine C
humanIF

Int J Cancer

Tissue-resident CXCR4+ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression

Authors - Zhenyu Liao
humanWB

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani