Anticorps Polyclonal de lapin anti-SP110

SP110 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), IP, ELISA, IF

Conjugaison

Non conjugué

Publications

1

N° de cat : 11502-1-AP

Synonymes

Speckled 110 kDa, SP110 nuclear body protein, IPR1, Interferon-induced protein 41/75, IFI75

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Galerie de données de validation

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  WB analysis using 11502-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11502-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11502-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11502-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11502-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11502-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11502-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11502-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 11502-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11502-1-AP

  IP result of anti-SP110 (IP:11502-1-AP, 4ug; Detection:11502-1-AP 1:800) with HeLa cells lysate 4000ug.

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  FC experiment of Jurkat using 11502-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SP110 (11502-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules COLO 320., cellules HeLa, cellules Jurkat, cellules K-562, cellules PC-3, tissu cérébral humain, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

11502-1-AP cible SP110 dans les applications de WB, FC (Intra), IP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SP110 Protéine recombinante Ag2042
• Nom complet: SP110 nuclear body protein
• Masse moléculaire calculée: 689 aa, 79 kDa
• Poids moléculaire observé: 62-66 kDa
• Numéro d’acquisition GenBank: BC019059
• Symbole du gène: SP110
• Identification du gène (NCBI): 3431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF,FC	J Clin Immunol; Detection of Sp110 by Flow Cytometry and Application to Screening Patients for Veno-occlusive Disease with Immunodeficiency.; Authors - Florian A Marquardsen

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011