Anticorps Polyclonal de lapin anti-SOX7

SOX7 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 23925-1-AP

Synonymes

SOX7, Transcription factor SOX 7

---

Galerie de données de validation

• 

  WB analysis using 23925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 23925-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 23925-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 23925-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human prostate cancer using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 23925-1-AP (SOX7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 23925-1-AP (SOX7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23925-1-AP (SOX7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23925-1-AP (SOX7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of PC-3 using 23925-1-AP

  Immunofluorescent analysis of PC-3 cells using 23925-1-AP (SOX7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules PC-3, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

23925-1-AP cible SOX7 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX7 Protéine recombinante Ag10323
• Nom complet: SRY (sex determining region Y)-box 7
• Masse moléculaire calculée: 388 aa, 42 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC071947
• Symbole du gène: SOX7
• Identification du gène (NCBI): 83595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SOX7, also named as Transcription factor SOX-7, is a 388 amino acid protein, which is widely expressed in adult and fetal tissues and tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation. SOX7 binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. SOX7 may be required for the survival of both hematopoietic and endothelial precursors during specification.

Publications

Species	Application	Title
human	WB	Oncogene; SOX7 regulates MAPK/ERK-BIM mediated apoptosis in cancer cells.; Authors - Qiao-Yang Sun
human	WB,IP	Mol Genet Genomics; Predisposition to atrioventricular septal defects may be caused by SOX7 variants that impair interaction with GATA4.; Authors - Baolei Li
human	WB	Cell Death Dis; The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.; Authors - Nanchao Hong