Anticorps Polyclonal de lapin anti-SOX6

SOX6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14010-1-AP

Synonymes

HSSOX6, SOX6, SOXD, Transcription factor SOX 6

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Galerie de données de validation

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  WB analysis using 14010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14010-1-AP (SOX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14010-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14010-1-AP (SOX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules C6, cellules Jurkat
• Résultats positifs en IHC: tissu testiculaire de rat, tissu cutané de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

14010-1-AP cible SOX6 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX6 Protéine recombinante Ag5111
• Nom complet: SRY (sex determining region Y)-box 6
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC047064
• Symbole du gène: SOX6
• Identification du gène (NCBI): 55553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Sex-determining Region Y (SRY)-related high-mobilitygroup box 6 (SOX6) is a transcription factor that was discovered in 1990s while searching for Sry-related genes. SOX6 belongs to the subfamily D of SOX protein and can recognize the conserved sequence of "(A/T)(A/T)CAA(A/ T)G" within the transcriptional regulation region of the target gene with its HMG domain. Sox6 contains DNA binding domain and binds to the minor groove of DNA. The ability of SOX6 to interact with different proteins at different places and times, makes it an intricate protein with roles in multiple processes. SOX6 has been implicated in diabetic nephropathy, adipogenesis, cardiomyopathy, inflammatory bowel disease, and cancer. (PMID: 33230925)

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	WB	Nat Commun; VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.; Authors - Yuki Miyamoto
mouse	WB	Int J Mol Med; miRNA‑342 suppresses renal interstitial fibrosis in diabetic nephropathy by targeting SOX6.; Authors - Zhen-Huan Jiang
human	WB	Nephrology (Carlton); CircHOMER1 aggravates oxidative stress, inflammation and ECM deposition in high glucose-induced human mesangial cells; Authors - Shi Shu
	IF	bioRxiv; Eed controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest; Authors - Tim Casey-Clyde
human	WB	Biochem Biophys Res Commun; MicroRNA-135a-5p promotes neuronal differentiation of pluripotent embryonal carcinoma cells by repressing Sox6/CD44 pathway.; Authors - Wang Li