- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-SOX2
SOX2 Polyclonal Antibody for WB, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, IF-P, ELISA
Conjugaison
Non conjugué
N° de cat : 20118-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu cérébral de souris, cellules HEK-293 transfectées, tissu cérébral de rat, tissu embryonnaire de souris |
| Résultats positifs en IHC | tissu embryonnaire de souris, tissu cérébral de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF-P | tissu cérébral de souris, tissu embryonnaire de souris |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 14 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
Informations sur le produit
20118-1-AP cible SOX2 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | Humain, poulet, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | SOX2 Protéine recombinante Ag13635 |
| Nom complet | SRY (sex determining region Y)-box 2 |
| Masse moléculaire calculée | 34 kDa |
| Poids moléculaire observé | 34-40 kDa |
| Numéro d’acquisition GenBank | BC013923 |
| Symbole du gène | SOX2 |
| Identification du gène (NCBI) | 6657 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Background
SRY (sex determining region Y)-box 2 is a transcription factor involved in neural stem cell maintenance and has been used in somatic cell reprogramming.
What is the molecular weight of Sox2?
34 kDa. Sox2 is an intronless gene encoding xx amino acids including HMG (High-mobility group) box domains that can bind to DNA.
What is the tissue specificity of Sox2?
In embryonic stem cells, Sox2 is highly expressed and is essential in self-renewal. In the developing embryo, Sox2 is found in the neural tube and then in the developing eye and central nervous system (CNS). This expression is highly conserved across organisms (PMID: 25126380; PMID: 8625802).
In adult tissues Sox2 is expressed in neural progenitor cells, which as multipotent cells can both self-renew and differentiate into neurons, astroglia, and oligodendroglia. It maintains their stem cell phenotype and is a useful marker of early neural stem cells (PMID: 16139372).
What is the role of Sox2 in pluripotency?
Sox2 acts as part of the LIF (Leukemia inhibitory factor) pathway, activated via JAK-STAT signaling to form complexes with other transcription factors such as Oct4 that bind to DNA and work to control pluripotency. Sox2 was described as one of the four original factors that are key to the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (PMID: 16904174).
What is the role of Sox2 in cancer?
The ability to control pluripotency means dysregulation of Sox2 can lead to uncontrolled proliferation. In many different cancers, Sox2 has been associated with the metastasis and growth of tumors, mostly due to overexpression (PMID: 28388544). The increased levels of Sox2 RNA and protein in tumor tissue highlight it as a potential therapeutic target (PMID: 25114775).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX2 antibody 20118-1-AP | Download protocol |
| IHC protocol for SOX2 antibody 20118-1-AP | Download protocol |
| IF protocol for SOX2 antibody 20118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). | ||
Int J Mol Sci Patients' Stem Cells Differentiation in a 3D Environment as a Promising Experimental Tool for the Study of Amyotrophic Lateral Sclerosis. | ||
Cell Death Dis Linc-DYNC2H1-4 promotes EMT and CSC phenotypes by acting as a sponge of miR-145 in pancreatic cancer cells. | ||
Sci Rep Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. | ||
Cell Cycle LncRNA SOX2OT affects cervical cancer cell growth, migration and invasion by regulating SOX2. | ||
J Cell Mol Med Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jacob (Verified Customer) (05-08-2023) | good signal in D20 old neuronal organoids
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FH Lisa (Verified Customer) (11-07-2022) | Proteintech is always a guarantee of good antibodies! The antibodies works very well for IF, not yet tested for WB.
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FH Ryan (Verified Customer) (02-14-2018) | NaCit antigen retrieval ph=6
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