Anticorps Polyclonal de lapin anti-SOX13

• Phare
• Validé par KD/KO

SOX13 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18902-1-AP

Synonymes

ICA12, Islet cell antigen 12, Sox 13, SOX13, Transcription factor SOX 13

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Galerie de données de validation

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  WB analysis using 18902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 18902-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 18902-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 18902-1-AP

  IP result of anti-SOX13 (IP:18902-1-AP, 4ug; Detection:18902-1-AP 1:500) with SH-SY5Y cells lysate 1200ug.

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  IF Staining of HeLa using 18902-1-AP

  Immunofluorescent analysis of Hela cells, using SOX13 antibody 18902-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules NCI-H1299
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

18902-1-AP cible SOX13 dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX13 Protéine recombinante Ag13537
• Nom complet: SRY (sex determining region Y)-box 13
• Masse moléculaire calculée: 622 aa, 69 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC106038
• Symbole du gène: SOX13
• Identification du gène (NCBI): 9580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRY-BOX 13(SOX13), identified a sequence homologous more than 60% to the SRY HMG box ,with a centrally located HMG box and an N-terminal leucine zipper motif with a neighboring glutamine stretch called a Q box. SOX13 binds to the consensus HMG-box motif. It's an IDDM-specific human autoantigen, and a gamma-delta-specific gene in the immune system. It can modulates Wnt/TCF activity. This is a rabbit polyclonal raised against part of N-terminal chain of SOX13 of human origin.

Publications

Species	Application	Title
human	WB,IF	Front Cardiovasc Med; Sox13 is a novel flow-sensitive transcription factor that prevents inflammation by repressing chemokine expression in endothelial cells; Authors - Catherine Demos ; KD Validated
human	WB,IHC	Lab Invest; Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1.; Authors - Min Feng ; KD Validated
mouse	WB, IF	Mol Cell Biochem; Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development.; Authors - Mikella Daigle
human	WB,IHC	Bioengineered; Sex-determining Region Y-box transcription factor 13 promotes breast cancer cell proliferation and glycolysis by activating the tripartite motif containing 11-mediated Wnt/β-catenin signaling pathway.; Authors - Xiaoyan Jin ; KD Validated
human	WB,chIP	J Exp Clin Cancer Res; FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.; Authors - Zhenwei He