Anticorps Polyclonal de lapin anti-SORLA

• Phare
• Validé par KD/KO

SORLA Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 22592-1-AP

Synonymes

C11orf32, LR11, LRP9, SORL1, SORLA, SorLA 1, Sortilin related receptor

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Galerie de données de validation

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  WB analysis of mouse brain using 22592-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22592-1-AP (SORLA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22592-1-AP

  IP result of anti-SORLA (IP:22592-1-AP, 5ug; Detection:22592-1-AP 1:500) with mouse brain tissue lysate 10000ug.

• 

  IHC staining of mouse brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22592-1-AP (SORLA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22592-1-AP (SORLA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22592-1-AP (SORLA antibody at dilution of 1:50.

• 

  IHC staining of human brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22592-1-AP (SORLA antibody at dilution of 1:50.

• 

  IHC staining of rat brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22592-1-AP (SORLA antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22592-1-AP (SORLA antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

22592-1-AP cible SORLA dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SORLA Protéine recombinante Ag18445
• Nom complet: sortilin-related receptor, L(DLR class) A repeats-containing
• Masse moléculaire calculée: 2214 aa, 248 kDa
• Poids moléculaire observé: 300 kDa
• Numéro d’acquisition GenBank: BC137171
• Symbole du gène: SORLA
• Identification du gène (NCBI): 6653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SorLA also known as SorL1 or LR11, is a multiligand receptor belonging to the LDL receptor superfamily. It is also a member of vacuolar protein sorting-10 (Vps10) family and is enriched in the brain. SorLA acts as an sorting receptor for APP and prevents amyloidogenic processing of APP. Downregulation of SorLA has been found in patients with sporadic Alzheimer disease (AD). Currently SorLA has emerged as a potential target for AD therapy. (25404298)

Publications

Species	Application	Title
mouse	WB, IP	Proc Natl Acad Sci U S A; α2A adrenergic receptor promotes amyloidogenesis through disrupting APP-SorLA interaction.; Authors - Yunjia Chen
mouse	WB	Environ Res; Exposure to a real traffic environment impairs brain cognition in aged mice; Authors - Chenlu Xu
human	WB,IHC,IF,CoIP	Funct Integr Genomics; SORL1 stabilizes ABCB1 to promote cisplatin resistance in ovarian cancer; Authors - Zhen Zheng ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: mouse brain tissue