Anticorps Polyclonal de lapin anti-Sorbitol dehydrogenase

• Phare
• Validé par KD/KO

Sorbitol dehydrogenase Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15881-1-AP

Synonymes

L iditol 2 dehydrogenase, SDH, Sorbitol dehydrogenase, SORD, SORD1

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Galerie de données de validation

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  WB analysis of HeLa using 15881-1-AP

  WB result of Sorbitol dehydrogenase antibody (15881-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Sorbitol dehydrogenase transfected HeLa cells.

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  WB analysis using 15881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15881-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15881-1-AP

  WB result of Sorbitol dehydrogenase antibody (15881-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Sorbitol dehydrogenase transfected HeLa cells.

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  WB analysis of human kidney using 15881-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15881-1-AP

  IP result of anti-Sorbitol dehydrogenase (IP:15881-1-AP, 4ug; Detection:15881-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu hépatique de rat, tissu hépatique de souris, tissu rénal humain
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

15881-1-AP cible Sorbitol dehydrogenase dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Sorbitol dehydrogenase Protéine recombinante Ag8651
• Nom complet: sorbitol dehydrogenase
• Masse moléculaire calculée: 357 aa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC021085
• Symbole du gène: SORD
• Identification du gène (NCBI): 6652
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Endogenous fructose production and metabolism in the liver contributes to the development of metabolic syndrome.; Authors - Lanaspa Miguel A MA
mouse	WB,IF	Reproduction; Nicotine reduces Sord expression and tyrosine phosphorylation in capacitated sperm.; Authors - Jingbo Dai
mouse	WB	J Mol Med (Berl); Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.; Authors - Yu Sun
rat	WB	Chem Biol Interact; Hepatocyte ferroptosis contributes to anti-tuberculosis drug-induced liver injury: Involvement of the HIF-1α/SLC7A11/GPx4 axis; Authors - Yining Liu
mouse	WB	Nutrients; Endogenous Fructose Production and Metabolism Drive Metabolic Dysregulation and Liver Disease in Mice with Hereditary Fructose Intolerance; Authors - Ana Andres-Hernando