Anticorps Polyclonal de lapin anti-SORBS2

• Phare
• Validé par KD/KO

SORBS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 24643-1-AP

Synonymes

Arg/Abl interacting protein 2, ARGBP2, KIAA0777, nArgBP2, PRO0618, Sorbin, SORBS2

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Galerie de données de validation

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  WB analysis using 24643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 24643-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 24643-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 24643-1-AP

  IP result of anti-SORBS2 (IP:24643-1-AP, 4ug; Detection:24643-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 24643-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 24643-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules MDCK, cellules U-251, tissu pancréatique de rat, tissu pancréatique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below

Informations sur le produit

24643-1-AP cible SORBS2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SORBS2 Protéine recombinante Ag20041
• Nom complet: sorbin and SH3 domain containing 2
• Masse moléculaire calculée: 1100 aa, 124 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC011883
• Symbole du gène: SORBS2
• Identification du gène (NCBI): 8470
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

Publications

Species	Application	Title
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	WB,IHC,IF,IP	EBioMedicine; Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.; Authors - Chunyan Li
human,mouse	WB,IP	Aging (Albany NY); The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.; Authors - Chunyan Li
human	WB, IP	J Biol Chem; Arg Kinase Binding Protein 2 (ArgBP2) interaction with α-actinin and actin stress fibres inhibits cell migration.; Authors - Praju Vikas Anekal
human	WB	J Transl Med; SORBS2 as a molecular target for atherosclerosis in patients with familial hypercholesterolemia.; Authors - Ming-Ming Liu
human	WB	Mol Med Rep; Musk ketone induces apoptosis of gastric cancer cells via downregulation of sorbin and SH3 domain containing 2.; Authors - Juan An ; KD Validated