Anticorps Polyclonal de lapin anti-SORBS1

• Phare
• Validé par KD/KO

SORBS1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13854-1-AP

Synonymes

c Cbl associated protein, CAP, Ponsin, R85FL, SH3 domain protein 5, SH3D5, SH3P12, SORB1, SORBS1

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Galerie de données de validation

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  WB analysis of 3T3-L1 using 13854-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13854-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13854-1-AP

  IP result of anti-SORBS1 (IP:13854-1-AP, 4ug; Detection:13854-1-AP 1:800) with mouse liver tissue lysate 4800ug.

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Applications testées

• Résultats positifs en WB: cellules 3T3-L1, cellules HEK-293, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

13854-1-AP cible SORBS1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SORBS1 Protéine recombinante Ag4828
• Nom complet: sorbin and SH3 domain containing 1
• Masse moléculaire calculée: 1292 aa, 142 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC042612
• Symbole du gène: SORBS1
• Identification du gène (NCBI): 10580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB，IHC	Front Cell Dev Biol; Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.; Authors - Yiyang Xu ; KD Validated
mouse	IF	Front Cell Neurosci; Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1.; Authors - Anchel González-Barriga
canine	IF	PLoS One; Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells.; Authors - Karin Fredriksson-Lidman
human,mouse	WB	Sci Rep; STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl and regulates adipocyte differentiation; Authors - Yuichi Sekine
mouse	WB	PLoS One; Proteomic changes in the hippocampus of large mammals after total-body low dose radiation; Authors - Diego Iacono