Anticorps Polyclonal de lapin anti-SNRPF

SNRPF Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14977-1-AP

Synonymes

PBSCF, Sm F, Sm protein F, SMF, snRNP F, SNRPF

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Galerie de données de validation

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  WB analysis of human heart using 14977-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14977-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14977-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14977-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14977-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14977-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14977-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14977-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 14977-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 14977-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 14977-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 14977-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14977-1-AP (SNRPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HeLa, cellules HepG2, cellules MCF-7, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14977-1-AP cible SNRPF dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRPF Protéine recombinante Ag6889
• Nom complet: small nuclear ribonucleoprotein polypeptide F
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC002505
• Symbole du gène: SNRPF
• Identification du gène (NCBI): 6636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB), D1 (SNRPD1), D2 (SNRPD2), D3 (SNRPD3), E (SNRPE), F (SNRPF), and G (SNRPG). These proteins have a role in the biogenesis of the snRNPs.