Anticorps Polyclonal de lapin anti-SNRPD2

SNRPD2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14789-1-AP

Synonymes

Sm D2, SMD2, snRNP core protein D2, SNRPD2

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Galerie de données de validation

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  WB analysis of A2780 using 14789-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 14789-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14789-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14789-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14789-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14789-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules A549, cellules HL-60
• Résultats positifs en IHC: tissu cutané humain, tissu de cancer du poumon humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14789-1-AP cible SNRPD2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRPD2 Protéine recombinante Ag6443
• Nom complet: small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14-16 kDa
• Numéro d’acquisition GenBank: BC000486
• Symbole du gène: SNRPD2
• Identification du gène (NCBI): 6633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.

Publications

Species	Application	Title
human	WB	RNA Biol; Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.; Authors - Sikandar Azam