Anticorps Polyclonal de lapin anti-SNRPA1

SNRPA1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17368-1-AP

Synonymes

SNRPA1, U2 snRNP A

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Galerie de données de validation

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  WB analysis of HeLa using 17368-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17368-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17368-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17368-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17368-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17368-1-AP

  IP result of anti-SNRPA1 (IP:17368-1-AP, 4ug; Detection:17368-1-AP 1:500) with HepG2 cells lysate 1000ug.

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  IHC staining of human lung using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17368-1-AP

  Immunofluorescent analysis of HepG2 cells, using SNRPA1 antibody 17368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules Raji
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cérébral humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

17368-1-AP cible SNRPA1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRPA1 Protéine recombinante Ag10790
• Nom complet: small nuclear ribonucleoprotein polypeptide A'
• Masse moléculaire calculée: 255 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC022816
• Symbole du gène: SNRPA1
• Identification du gène (NCBI): 6627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP.; Authors - Yajie Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Cell Rep; SF3B1 Association with Chromatin Determines Splicing Outcomes.; Authors - Nir Kfir
human	WB	STAR Protoc; Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.; Authors - Li Wang
human	WB,IHC	Clin Respir J; High SNRPA1 expression leads to poor prognosis in patients with lung adenocarcinoma; Authors - Juan Juan Yang