Anticorps Polyclonal de lapin anti-SNRPA

• Phare
• Validé par KD/KO

SNRPA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (2)

N° de cat : 10212-1-AP

Synonymes

SNRPA, U1 A, U1 snRNP A, U1A

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Galerie de données de validation

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  WB analysis using 10212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10212-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10212-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10212-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10212-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10212-1-AP (SNRPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10212-1-AP

  IP result of anti-SNRPA (IP:10212-1-AP, 4ug; Detection:10212-1-AP 1:500) with HEK-293 cells lysate 2000 ug.

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  IHC staining of human cervical cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10212-1-AP (SNRPA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10212-1-AP (SNRPA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10212-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10212-1-AP (SNRPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10212-1-AP (SNRPA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer de l'estomac humain, tissu de côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

10212-1-AP cible SNRPA dans les applications de WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: canin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRPA Protéine recombinante Ag0283
• Nom complet: small nuclear ribonucleoprotein polypeptide A
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC000405
• Symbole du gène: SNRPA
• Identification du gène (NCBI): 6626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.

Publications

Species	Application	Title
human	WB,ChIP	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB,IHC,IF	Cell Prolif; SNRPA enhances tumour cell growth in gastric cancer through modulating NGF expression.; Authors - Ning Dou ; KD Validated
canine	WB	Biochem Biophys Res Commun; Basolateral sorting of the Mg²⁺ transporter CNNM4 requires interaction with AP-1A and AP-1B.; Authors - Yusuke Hirata ; KD Validated
human	WB	Front Oncol; Designer Exosomes for Targeted Delivery of a Novel Therapeutic Cargo to Enhance Sorafenib-Mediated Ferroptosis in Hepatocellular Carcinoma; Authors - Xiaoju Li
human	WB	RNA Biol; Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.; Authors - Sikandar Azam
human	RIP	Mol Cell; Transcription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape; Authors - Prajwal C Boddu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

changwei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa S3

FH

Yaroslav (Verified Customer) (01-06-2020)

Specific signal with modest sensitivity at current dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse ES cells

SNRPA Antibody Western Blot, validation (1:1000 dilution) in mouse ES cells (Cat no:10212-1-AP)