Anticorps Polyclonal de lapin anti-SNRNP200

SNRNP200 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 23875-1-AP

Synonymes

KIAA0788, HELIC2, EC:3.6.4.13, BRR2, ASCC3L1

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Galerie de données de validation

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  WB analysis of HeLa using 23875-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23875-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23875-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23875-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 23875-1-AP

  IP result of anti-SNRNP200 (IP:23875-1-AP, 4ug; Detection:23875-1-AP 1:1000) with HeLa cells lysate 4000ug.

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  IHC staining of mouse testis using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 23875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 23875-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human SNRNP200 (23875-1-AP) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain, tissu d'estomac de rat, tissu testiculaire de souris, tissu vésical de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

23875-1-AP cible SNRNP200 dans les applications de WB, IHC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNRNP200 Protéine recombinante Ag20748
• Nom complet: small nuclear ribonucleoprotein 200kDa (U5)
• Masse moléculaire calculée: 494 aa, 57 kDa
• Poids moléculaire observé: 200-245 kDa
• Numéro d’acquisition GenBank: BC001417
• Symbole du gène: SNRNP200
• Identification du gène (NCBI): 23020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
human	IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Works fine by WB to detect SNRNP200 from HeLa extracts (1/1000 dilution, overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa