Anticorps Polyclonal de lapin anti-Syntaphilin

Syntaphilin Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 13646-1-AP

Synonymes

KIAA0374, SNPH, syntaphilin

---

Galerie de données de validation

• 

  WB analysis using 13646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13646-1-AP (Syntaphilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse trachea using 13646-1-AP

  mouse trachea tissue were subjected to SDS PAGE followed by western blot with 13646-1-AP (Syntaphilin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13646-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13646-1-AP (Syntaphilin antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13646-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13646-1-AP (Syntaphilin antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de trachée de souris
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13646-1-AP cible Syntaphilin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syntaphilin Protéine recombinante Ag4594
• Nom complet: syntaphilin
• Masse moléculaire calculée: 538 aa, 58 kDa
• Poids moléculaire observé: 70-75 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC035788
• Symbole du gène: SNPH
• Identification du gène (NCBI): 9751
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syntaphilin is a protein selectively expressed in brain. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits SNARE complex formation by absorbing free syntaxin-1, and thereby regulates synaptic vesicle exocytosis (PMID: 10707983). The deduced 537-amino acid syntaphilin protein contains an N-terminal proline-rich domain, a predicted coiled-coil domain, a putative C-terminal transmembrane domain, and numerous consensus sites for protein phosphorylation. Whereas the calculated molecular mass of syntaphilin is 58 kDa, the apparent molecular mass of 70-75 kDa is larger than the predicted mass, suggesting a posttranslational modification of syntaphilin (PMID: 9205841; 10707983). An extra band of 65 kDa was also detected by this antibody that may represent an alternative syntaphilin gene product or a stable degradation product (PMID: 10707983; 24457644).

Publications

Species	Application	Title
rat	IF	Sci Rep; Identification of a novel interaction of FUS and syntaphilin may explain synaptic and mitochondrial abnormalities caused by ALS mutations.; Authors - Shaakir Salam
mouse	WB	Mol Brain; Disrupted-in-schizophrenia 1 (DISC1) and Syntaphilin collaborate to modulate axonal mitochondrial anchoring.; Authors - Cana Park
rat	WB	Brain Res; Miro1 provides neuroprotection via the mitochondrial trafficking pathway in a rat model of traumatic brain injury.; Authors - Chen Chen
rat	WB	Bioeng Transl Med; Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36; Authors - Zhijie Zhu
mouse	WB	Neuroscience; Armcx1 reduces neurological damage via a mitochondrial transport pathway involving Miro1 after traumatic brain injury; Authors - Qiuying Li
mouse	IHC	Neurochem Int; 3-Nitrotyrosine shortens axons of non-dopaminergic neurons by inhibiting mitochondrial motility; Authors - Masahiro Hirai

{{ptg:RelatedPrimaryAntibodies}}