Anticorps Polyclonal de lapin anti-RCC1

• Phare
• Validé par KD/KO

RCC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 10697-1-AP

Synonymes

RCC1, SNHG3 RCC1, SNHG3-RCC1

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Galerie de données de validation

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  WB analysis of HEK-293 using 10697-1-AP

  WB result of RCC1 antibody (10697-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCC1 transfected HEK-293 cells.

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  WB analysis using 10697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10697-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10697-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10697-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10697-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10697-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10697-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10697-1-AP

  IP result of anti-RCC1 (IP:10697-1-AP, 4ug; Detection:10697-1-AP 1:500) with HeLa cells lysate 1600 ug.

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  IHC staining of human ovary tumor using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules Raji, cellules SGC-7901, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10697-1-AP cible RCC1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RCC1 Protéine recombinante Ag1042
• Nom complet: SNHG3-RCC1 readthrough transcript
• Masse moléculaire calculée: 45 kDa, 48 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC007300
• Symbole du gène: RCC1
• Identification du gène (NCBI): 751867
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.