Anticorps Polyclonal de lapin anti-SMYD1-Specific

• Phare
• Validé par KD/KO

SMYD1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 16151-1-AP

Synonymes

BOP, SMYD1, SMYD1-Specific, ZMYND18, ZMYND22

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Galerie de données de validation

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  WB analysis of mouse heart using 16151-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16151-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16151-1-AP

  IP result of anti-SMYD1-Specific (IP:16151-1-AP, 4ug; Detection:16151-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human testis using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque de souris, tissu cardiaque humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16151-1-AP cible SMYD1-Specific dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SET and MYND domain containing 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: NM_198274
• Symbole du gène: SMYD1
• Identification du gène (NCBI): 150572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Toxicol Lett; Down-regulated Smyd1 participated in the inhibition of myoblast differentiation induced by cigarette smoke extract; Authors - Fang Li ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

SMYD1 antibody worked very well in human differentiated cardiomyocyte.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Human cardiomyocytes

FH

Eric (Verified Customer) (10-15-2018)

Very low detection of SMYD1: 45 ug protein, overnight incubation with primary antibody at 4 oC, 2h incubation with secondary at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: C2C12/mouse heart