Anticorps Polyclonal de lapin anti-SMU1

SMU1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 15511-1-AP

Synonymes

BWD, fSAP57, RP11 54K16.3, SMU 1, SMU1

---

Galerie de données de validation

• 

  WB analysis of mouse liver using 15511-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15511-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15511-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMU1 antibody 15511-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules K-562
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain, tissu de cirrhose hépatique humain, tissu ovarien humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15511-1-AP cible SMU1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMU1 Protéine recombinante Ag7688
• Nom complet: smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC002876
• Symbole du gène: SMU1
• Identification du gène (NCBI): 55234
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sum1, belongs to the WD repeat SMU1 family. Human SMU1 is a component of the spliceosome fSAP57(PMID: 15254247). It is possible that the mutation in Smu1 affects spliceosome function, resulting in generation of improper splice variants, which could explain the phenotypes of tsTM18 cells. Some results suggest that Smu1 participates in at least two steps of splicing: splice-site choice and control of accuracy(PMID: 17105761). This antibody is a rabbit polyclonal antibody raised against a region of human REDD1 antigen.