Anticorps Polyclonal de lapin anti-SMPD1,ASM

• Phare
• Validé par KD/KO

SMPD1,ASM Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 14609-1-AP

Synonymes

ASM, SMPD1, SMPD1,ASM

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Galerie de données de validation

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  WB analysis using 14609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14609-1-AP (SMPD1,ASM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

14609-1-AP cible SMPD1,ASM dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMPD1,ASM Protéine recombinante Ag6180
• Nom complet: sphingomyelin phosphodiesterase 1, acid lysosomal
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 60 kDa, 70-75 kDa
• Numéro d’acquisition GenBank: BC041164
• Symbole du gène: SMPD1
• Identification du gène (NCBI): 6609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.

Publications

Species	Application	Title
mouse		Cell Host Microbe; The β2Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.; Authors - Alexander Gluschko ; KO Validated
human	WB,IF	Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang
mouse	IF	Acta Neuropathol Commun; Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse; Authors - Simone M Crivelli
mouse	IF	Cells; Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease; Authors - Ahmed Elsherbini
human	IHC	J Mol Med (Berl); Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma; Authors - Gregory C Wilson

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alice (Verified Customer) (05-11-2021)

Good Ab should show good result in your website to convince people to use it.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Pancreas

SMPD1,ASM Antibody Immunohistochemistry validation (1:200 dilution) in Mouse Pancreas (Cat no:14609-1-AP)