Anticorps Polyclonal de lapin anti-SMOX

• Phare
• Validé par KD/KO

SMOX Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 15052-1-AP

Synonymes

C20orf16, PAO, PAO 1, PAOh1, Polyamine oxidase 1, SMO, SMOX, spermine oxidase

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Galerie de données de validation

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  WB analysis of PC-13 using 15052-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15052-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules MCF-7, cellules PC-13, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer de l'estomac humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

15052-1-AP cible SMOX dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMOX Protéine recombinante Ag7035
• Nom complet: spermine oxidase
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 56-65 kDa
• Numéro d’acquisition GenBank: BC000669
• Symbole du gène: SMOX
• Identification du gène (NCBI): 54498
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.

Publications

Species	Application	Title
human	WB	J Clin Invest; Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma.; Authors - Anna Bianchi-Smiraglia
mouse	WB	J Clin Invest; Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.; Authors - Leslie A Sandusky-Beltran
mouse	WB	EBioMedicine; Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.; Authors - Guangtian Wang
human	WB	J Invest Dermatol; The Polyamine Putrescine Promotes Human Epidermal Melanogenesis.; Authors - Aishwarya Sridharan
		Free Radic Biol Med; Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: Evaluation of a new transgenic mouse model.; Authors - Roberta Ceci
human	WB,IHC	Biomedicines; Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients.; Authors - Sooyoun Kim