Anticorps Polyclonal de lapin anti-SMO

SMO Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (1)

N° de cat : 20787-1-AP

Synonymes

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Galerie de données de validation

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  IHC staining of human prostate cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20787-1-AP (SMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20787-1-AP (SMO Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 5 publications below
• IF: See 6 publications below

Informations sur le produit

20787-1-AP cible SMO dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMO Protéine recombinante Ag14322
• Nom complet: smoothened homolog (Drosophila)
• Masse moléculaire calculée: 787 aa, 86 kDa
• Numéro d’acquisition GenBank: BC009989
• Symbole du gène: SMO
• Identification du gène (NCBI): 6608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays a central role in animal development and stem-cell function. Defects in Hh signaling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Publications

Species	Application	Title
human,mouse		Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
chicken	IF	Sci Adv; Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.; Authors - Gabriela Toro-Tapia
human	IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	IF	J Cell Biol; Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.; Authors - Sarah Findakly
human	IF	Acta Neuropathol Commun; Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals.; Authors - Sarah Findakly
human		Cell Death Discov; N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness; Authors - Hongliang Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (05-13-2019)

For IF, it could label the ciliary localisation of endogenous Smo; for WB, it could label many bands around 100 kd.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF