Anticorps Polyclonal de lapin anti-SMC4

• Phare
• Validé par KD/KO

SMC4 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 24758-1-AP

Synonymes

CAPC, hCAP C, SMC 4, SMC protein 4, SMC4, SMC4L1, XCAP C homolog

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Galerie de données de validation

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  WB analysis of MCF-7 using 24758-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24758-1-AP (SMC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24758-1-AP

  Immunofluorescent analysis of HepG2 cells using 24758-1-AP (SMC4 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IHC: tissu de côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

24758-1-AP cible SMC4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMC4 Protéine recombinante Ag19156
• Nom complet: structural maintenance of chromosomes 4
• Masse moléculaire calculée: 1288 aa, 147 kDa
• Poids moléculaire observé: 147-150 kDa
• Numéro d’acquisition GenBank: BC106033
• Symbole du gène: SMC4
• Identification du gène (NCBI): 10051
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures.; Authors - Ipek Ilgin Gönenc
rat	IHC	Gene; Transcriptional profiling of rat skeletal muscle hypertrophy under restriction of blood flow.; Authors - Shouyu Xu
		Sci Rep; Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.; Authors - Johan Busselez
human,mouse	WB,IHC,IF	Cell Metab; The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity; Authors - Xuedan Sun ; KO Validated