Anticorps Polyclonal de lapin anti-SMARCE1

SMARCE1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 10814-1-AP

Synonymes

BAF57, BRG1 associated factor 57, SMARCE1

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Galerie de données de validation

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  WB analysis of Jurkat using 10814-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10814-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10814-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10814-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10814-1-AP

  IP result of anti-SMARCE1 (IP:10814-1-AP, 4ug; Detection:10814-1-AP 1:800) with HEK-293 cells lysate 880ug.

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  IHC staining of human prostate cancer using 10814-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10814-1-AP (SMARCE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10814-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10814-1-AP (SMARCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10814-1-AP cible SMARCE1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMARCE1 Protéine recombinante Ag1161
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007082
• Symbole du gène: SMARCE1
• Identification du gène (NCBI): 6605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.

Publications

Species	Application	Title
mouse	WB	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song