Anticorps Monoclonal anti-SMAD7

SMAD7 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

2B9A4

N° de cat : 66478-1-Ig

Synonymes

CRCS3, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, SMAD 7, SMAD family member 7, SMAD7

---

Galerie de données de validation

• 

  WB analysis using 66478-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66478-1-Ig (SMAD7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 66478-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66478-1-Ig (SMAD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 66478-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66478-1-Ig (SMAD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66478-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66478-1-Ig (SMAD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66478-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66478-1-Ig (SMAD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu rénal de porc, tissu rénal de rat
• Résultats positifs en IHC: tissu de cervelet de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below

Informations sur le produit

66478-1-Ig cible SMAD7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMAD7 Protéine recombinante Ag13688
• Nom complet: SMAD family member 7
• Masse moléculaire calculée: 426 aa, 46 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC074819
• Symbole du gène: SMAD7
• Identification du gène (NCBI): 4092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Publications

Species	Application	Title
human,mouse	WB	Acta Pharmacol Sin; The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.; Authors - Yang Sun ; KD Validated
human	WB	Aging (Albany NY); LINC00968 can inhibit the progression of lung adenocarcinoma through the miR-21-5p/SMAD7 signal axis.; Authors - Yuxing Zhu
rat	WB	Biomed Pharmacother; Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.; Authors - Yu Xia
mouse	WB	J Ethnopharmacol; Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation.; Authors - Fan Wu
human	WB	Exp Cell Res; Exosomes derived from thymic stromal lymphopoietin-treated dendritic cells regulate T helper 17/regulatory T cell differentiation via miR-21/Smad7 axis.; Authors - Qingshan Ji
rat	WB	Biochem Biophys Res Commun; Emodin ameliorates tubulointerstitial fibrosis in obstructed kidneys by inhibiting EZH2.; Authors - Lin Xu

Produits associés

TGF Beta 1 Polyclonal antibody

18978-1-AP

SMAD4 Polyclonal antibody

10231-1-AP

SMAD3 Polyclonal antibody

25494-1-AP

SMAD2 Polyclonal antibody

12570-1-AP

SMURF1 Polyclonal antibody

55175-1-AP

SMAD1 Polyclonal antibody

10429-1-AP