Anticorps Polyclonal de lapin anti-SMAD4

• Phare
• Validé par KD/KO

SMAD4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (5)

Applications

WB, IHC, IP, CoIP, ChIP, ELISA, IF

Conjugaison

Non conjugué

Publications

88

N° de cat : 10231-1-AP

Synonymes

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

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Galerie de données de validation

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  WB analysis using 10231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10231-1-AP

  WB result of SMAD4 antibody (10231-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD4 transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 10231-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10231-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10231-1-AP

  IP result of anti-SMAD4 (IP:10231-1-AP, 4ug; Detection:10231-1-AP 1:1500) with HeLa cells lysate 1320 ug.

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  IHC staining of human placenta using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HCT 116, cellules HEK-293, cellules HEK-293T, cellules Neuro-2a, cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 76 publications below
• IHC: See 10 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Informations sur le produit

10231-1-AP cible SMAD4 dans les applications de WB, IHC, IP, CoIP, ChIP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMAD4 Protéine recombinante Ag0299
• Nom complet: SMAD family member 4
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC002379
• Symbole du gène: SMAD4
• Identification du gène (NCBI): 4089
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

Publications

Species	Application	Title
human	WB	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB,IP,IF	Transl Res; Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.; Authors - Xinxin Liao ; KD Validated
human	WB	Theranostics; PMID: 28839463; Authors - Junlong Zhuang
rat	WB,IF	Acta Pharmacol Sin; Role of thrombospondin-1 in high-salt-induced mesenteric artery endothelial impairment in rats; Authors - Fang-Fang Xu ; KD Validated
human	WB	EMBO Rep; Mouse endothelial OTUD1 promotes angiotensin II-induced vascular remodeling by deubiquitinating SMAD3; Authors - Zhuqi Huang
human	WB	Stem Cell Reports; Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.; Authors - Wen-Li Deng