Anticorps Polyclonal de lapin anti-SMAD3

• Phare
• Validé par KD/KO

SMAD3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

71

N° de cat : 25494-1-AP

Synonymes

hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

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Galerie de données de validation

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  WB analysis of Jurkat using 25494-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 25494-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25494-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25494-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 25494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 25494-1-AP

  Immunofluorescent analysis of HeLa cells using 25494-1-AP (SMAD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules A549, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 65 publications below
• IHC: See 9 publications below
• IF: See 3 publications below

Informations sur le produit

25494-1-AP cible SMAD3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 3
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: NM_005902
• Symbole du gène: SMAD3
• Identification du gène (NCBI): 4088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

Publications

Species	Application	Title
mouse	IF	Circulation; Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation.; Authors - Yoko Kojima
human	WB	Cell Death Differ; REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.; Authors - Chan Jiao
human	WB	Oncogene; The β-catenin/TCF-4-LINC01278-miR-1258-Smad2/3 axis promotes hepatocellular carcinoma metastasis.; Authors - Wei-Juan Huang ; KD Validated
human	WB,IHC,IF	Stem Cell Reports; Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.; Authors - Wen-Li Deng ; KD Validated
rat	WB	Oxid Med Cell Longev; WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.; Authors - Xiaoyu Zhou
rat	WB	Int J Nanomedicine; A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway.; Authors - Ba Hieu Tran

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