Anticorps Polyclonal de lapin anti-SLU7

• Phare
• Validé par KD/KO

SLU7 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, RIP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12050-1-AP

Synonymes

9G8, hSlu7, Pre mRNA splicing factor SLU7, SLU7

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Galerie de données de validation

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  WB analysis of Jurkat using 12050-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis using 12050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12050-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12050-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12050-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12050-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12050-1-AP (SLU7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 12050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, cellules Y79, tissu de côlon de souris
• Résultats positifs en IHC: tissu pancréatique de souris, tissu de cancer du côlon humain, tissu d'estomac de rat, tissu pancréatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

12050-1-AP cible SLU7 dans les applications de WB, IF, RIP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLU7 Protéine recombinante Ag2680
• Nom complet: SLU7 splicing factor homolog (S. cerevisiae)
• Masse moléculaire calculée: 586 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC010634
• Symbole du gène: SLU7
• Identification du gène (NCBI): 10569
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pre-mRNA splicing occurs in two sequential transesterification steps. SLU7 is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions.

Publications

Species	Application	Title
human	RIP	Mol Cancer; A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer; Authors - Runjie Song
human,mouse	WB,IHC,IF	Adv Sci (Weinh); G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer; Authors - Xianchong Zheng ; KD Validated