Anticorps Polyclonal de lapin anti-SLK

SLK Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 19743-1-AP

Synonymes

CTCL tumor antigen se20 9, hSLK, KIAA0204, LOSK, se20 9, SLK, STE20 like kinase, STE20 like kinase (yeast), STE20 related kinase, STK2

---

Galerie de données de validation

• 

  WB analysis using 19743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19743-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19743-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19743-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 19743-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLK antibody (19743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, cellules THP-1, tissu cérébral humain, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19743-1-AP cible SLK dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: STE20-like kinase (yeast)
• Masse moléculaire calculée: 143 kDa
• Poids moléculaire observé: 143-160 kDa, 210-220 kDa
• Numéro d’acquisition GenBank: NM_014720
• Symbole du gène: SLK
• Identification du gène (NCBI): 9748
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)