Anticorps Polyclonal de lapin anti-NHE8

• Phare
• Validé par KD/KO

NHE8 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 18318-1-AP

Synonymes

KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8

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Galerie de données de validation

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  WB analysis using 18318-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18318-1-AP

  WB result of NHE8 antibody (18318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NHE8 transfected HEK-293 cells.

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  WB analysis of mouse liver using 18318-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18318-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 18318-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 18318-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 18318-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18318-1-AP (NHE8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu de côlon de souris, tissu hépatique de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cutané humain, tissu de côlon humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18318-1-AP cible NHE8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NHE8 Protéine recombinante Ag13111
• Nom complet: solute carrier family 9 (sodium/hydrogen exchanger), member 8
• Masse moléculaire calculée: 581 aa, 65 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC112213
• Symbole du gène: SLC9A8
• Identification du gène (NCBI): 23315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NHE8, encoded by SLC9A8, is a member of sodium/hydrogen exchanger family which are transmembrane proteins that mediate the electroneutral exchange of sodium (Na) for hydrogen (H) and get involved in intracellular pH homeostasis, cell volume regulation, acid-base regulation, and so on. NHE8 has a broad tissue distribution, with relatively high abundance in the gastrointestinal tract. It has been reported that NHE8 expression differs among different regions in the stomach, very low in nonglandular region whereas very high in glandular region. The predicted molecular weight of NHE8 is 64-65 kDa, while its glycosylated form often migrates around 70-85 kDa.