Anticorps Polyclonal de lapin anti-SLC35A1

• Phare
• Validé par KD/KO

SLC35A1 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16342-1-AP

Synonymes

CMP SA Tr, CMP Sia Tr, CMP sialic acid transporter, CMPST, CST, hCST, inactive, SLC35A1

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Galerie de données de validation

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  IHC staining of mouse spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16342-1-AP (SLC35A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16342-1-AP (SLC35A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16342-1-AP (SLC35A1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16342-1-AP (SLC35A1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC35A1 antibody (16342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, tissu cutané humain, tissu splénique humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16342-1-AP cible SLC35A1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC35A1 Protéine recombinante Ag9524
• Nom complet: solute carrier family 35 (CMP-sialic acid transporter), member A1
• Masse moléculaire calculée: 337 aa, 37 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC017807
• Symbole du gène: SLC35A1
• Identification du gène (NCBI): 10559
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Solute carrier family 35 member A1 (SLC35A1) , also named as CMP-sialic acid transporter (CST) , is responsible for transferring CMP-sialic acid from the cytosol through Golgi membranes, thus playing a vital role in the terminal sialylation of glycan structures. Slc35a1 deficiency causes thrombocytopenia due to impaired megakaryocytopoiesis and excessive platelet clearance in the liver. In addition, SCL35A1 acts independently from sialic acid in functional α-DG O-mannosylation(PMID: 25552652).

Publications

Species	Application	Title
human	IHC	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
human	WB, IF	Cells; Knockout of the CMP-Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays.; Authors - Antje Banning ; KO Validated
human	WB	Cell Rep; A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection.; Authors - Jessie Kulsuptrakul ; KO Validated