Anticorps Polyclonal de lapin anti-VGAT

VGAT Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (3)

N° de cat : 14471-1-AP

Synonymes

GABA and glycine transporter, hVIAAT, SLC32A1, Vesicular GABA transporter, VGAT, VIAAT

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Galerie de données de validation

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  WB analysis of mouse brain using 14471-1-AP

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14471-1-AP

  IP result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:5000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of rat brain using 14471-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14471-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 14471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris non bouilli,
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

14471-1-AP cible VGAT dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Caenorhabditis elegans
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VGAT Protéine recombinante Ag5843
• Nom complet: solute carrier family 32 (GABA vesicular transporter), member 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC053582
• Symbole du gène: VGAT
• Identification du gène (NCBI): 140679
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.

Publications

Species	Application	Title
mouse	WB	Acta Neuropathol Commun; Mutation-induced loss of APP function causes GABAergic depletion in recessive familial Alzheimer's disease: analysis of Osaka mutation-knockin mice.; Authors - Tomohiro Umeda
human	WB	Biochem J; Role of N-glycosylation in renal betaine transport.; Authors - Eva S Schweikhard
Caenorhabditis elegans	IHC,IF	PLoS One; Mutant TDP-43 and FUS cause age-dependent paralysis and neurodegeneration in C. elegans.; Authors - Vaccaro Alexandra A
human	IF	Curr Issues Mol Biol; miR-92a-2-5p Regulates the Proliferation and Differentiation of ASD-Derived Neural Progenitor Cells.; Authors - Wenting Zhuang
mouse	WB	Biol Trace Elem Res; Exercise Ameliorates Fluoride-induced Anxiety- and Depression-like Behavior in Mice: Role of GABA.; Authors - Qiqi Cao
mouse	IF	Exp Anim; TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models; Authors - Haiqing Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-04-2024)

vGAT worked on my human brain FFPE sections although the quantification would be difficult due to the high autofluorescence

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

VGAT Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:14471-1-AP)

FH

Mandi (Verified Customer) (03-10-2020)

Did not stain the nerve terminals well. Signal was localized to the soma mainly.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human cortical neurons

FH

Ute (Verified Customer) (03-03-2020)

blocking solution 5% skimmed milk in 1x TBS-T

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse hippocampal lysat