Anticorps Polyclonal de lapin anti-FATP4

• Phare
• Validé par KD/KO

FATP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 11013-1-AP

Synonymes

ACSVL4, FATP 4, FATP4, Fatty acid transport protein 4, SLC27A4

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Galerie de données de validation

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  WB analysis of HepG2 using 11013-1-AP

  WB result of FATP4 antibody (11013-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FATP4 transfected HepG2 cells.

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  WB analysis of human colon using 11013-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11013-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11013-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 11013-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11013-1-AP

  IP result of anti-FATP4 (IP:11013-1-AP, 4ug; Detection:11013-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11013-1-AP (FATP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules SMMC-7721, tissu cardiaque humain, tissu de côlon de rat, tissu de côlon de souris, tissu de côlon humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11013-1-AP cible FATP4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FATP4 Protéine recombinante Ag1490
• Nom complet: solute carrier family 27 (fatty acid transporter), member 4
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC009959
• Symbole du gène: FATP4/SLC27A4
• Identification du gène (NCBI): 10999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Sci Rep; PMID: 28304381; Authors - Wenjing Li
human	WB	Front Immunol; Cathelicidin-WA Facilitated Intestinal Fatty Acid Absorption Through Enhancing PPAR-γ Dependent Barrier Function.; Authors - Xin Zong
mouse	WB	iScience; Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.; Authors - Hu Hua
	WB	Int J Mol Sci; Nano-Zn Increased Zn Accumulation and Triglyceride Content by Up-Regulating Lipogenesis in Freshwater Teleost, Yellow Catfish Pelteobagrus fulvidraco.; Authors - Shi-Cheng Ling
mouse	IHC	Biochim Biophys Acta; Uninephrectomy augments the effects of high fat diet induced obesity on gene expression in mouse kidney.; Authors - Zhibo Gai
mouse,pig	WB	Cells; LPS Inhibits Fatty Acid Absorption in Enterocytes through TNF-α Secreted by Macrophages.; Authors - Heyuan Liu