Anticorps Polyclonal de lapin anti-ANT1/2

ANT1/2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 17796-1-AP

Synonymes

2F1, AAC2, ADP,ATP carrier protein 2, ADP/ATP translocase 2, ANT 2, ANT1/2, SLC25A5, T2, T3

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Galerie de données de validation

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  WB analysis using 17796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17796-1-AP (ANT1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque de souris, tissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de cancer du foie humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 5 publications below

Informations sur le produit

17796-1-AP cible ANT1/2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANT1/2 Protéine recombinante Ag11956
• Nom complet: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Masse moléculaire calculée: 298 aa, 33 kDa
• Poids moléculaire observé: 30-32 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC056160
• Symbole du gène: SLC25A5
• Identification du gène (NCBI): 292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)

Publications

Species	Application	Title
	WB	Elife; Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases.; Authors - Jiaqi Fu
mouse	WB	PLoS Genet; Novel insights into SLC25A46-related pathologies in a genetic mouse model.; Authors - Maria Eirini Terzenidou
human	IHC	Mol Cell Proteomics; Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans.; Authors - Kathleen C Lundberg
rat	WB	Exp Neurol; Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder.; Authors - Jin-Hee Seo
mouse	WB	J Proteome Res; Proteomic Identification of the SLC25A46 Interactome in Transgenic Mice Expressing SLC25A46-FLAG.; Authors - Vasiliki-Iris Perivolidi
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang