Anticorps Polyclonal de lapin anti-SLC25A15

SLC25A15 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 15317-1-AP

Synonymes

D13S327, HHH, ORC1, ORNT1, SLC25A15

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Galerie de données de validation

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  WB analysis of HeLa using 15317-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15317-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 15317-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15317-1-AP (SLC25A15 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15317-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody (15317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu d'estomac de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15317-1-AP cible SLC25A15 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC25A15 Protéine recombinante Ag7563
• Nom complet: solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC002702
• Symbole du gène: SLC25A15
• Identification du gène (NCBI): 10166
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa.