Anticorps Polyclonal de lapin anti-SLC25A10

• Phare
• Validé par KD/KO

SLC25A10 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12086-1-AP

Synonymes

DIC, SLC25A10

---

Galerie de données de validation

• 

  WB analysis using 12086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12086-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12086-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12086-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12086-1-AP

  IP result of anti-SLC25A10 (IP:12086-1-AP, 3ug; Detection:12086-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human lung using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HepG2, cellules PC-3, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

12086-1-AP cible SLC25A10 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC25A10 Protéine recombinante Ag2719
• Nom complet: solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
• Masse moléculaire calculée: 296 aa, 32 kDa
• Poids moléculaire observé: 29-31 kDa
• Numéro d’acquisition GenBank: BC015797
• Symbole du gène: SLC25A10
• Identification du gène (NCBI): 1468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC25A10, also known as dicarboxylate ion carrier (DIC), is a mitochondrial inner membrane carrier protein which catalyzes the transport of dicarboxylates such as malate and succinate across the mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate. SLC25A10 is expressed at highest levels in kidney and liver, lower levels in lung, spleen, heart, and brain, and lowest levels in skeletal muscle. Two isoforms of SLC25A10 exist due to the alternative splicing.

Publications

Species	Application	Title
human	IHC,WB	Hum Cell; The role of PYCR1 in inhibiting 5-fluorouracil-induced ferroptosis and apoptosis through SLC25A10 in colorectal cancer; Authors - Borong Zhou
human	WB,IHC	Oncol Lett; SLC25A10 performs an oncogenic role in human osteosarcoma.; Authors - Gaoyuan Wang ; KD Validated
human,rat	WB,IF,IHC	J Cardiovasc Transl Res; Mild Therapeutic Hypothermia Alleviated Myocardial Ischemia/Reperfusion Injury via Targeting SLC25A10 to Suppress Mitochondrial Apoptosis; Authors - Senlin Ma