Anticorps Polyclonal de lapin anti-SLC24A4

• Phare
• Validé par KD/KO

SLC24A4 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 18992-1-AP

Synonymes

NCKX4, SHEP6, SLC24A2, SLC24A4

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Galerie de données de validation

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  WB analysis using 18992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 18992-1-AP

  WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.

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  WB analysis of mouse brain using 18992-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 18992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 18992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

18992-1-AP cible SLC24A4 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 62-90 kDa
• Numéro d’acquisition GenBank: NM_153646
• Symbole du gène: SLC24A4
• Identification du gène (NCBI): 123041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

Publications

Species	Application	Title
mouse	IHC	Bone; Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.; Authors - Akane Inoue
rat	WB	J Cell Physiol; Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.; Authors - Lacruz Rodrigo S RS
mouse	WB	Calcif Tissue Int; Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.; Authors - A L J J Bronckers
mouse	WB	Cells Tissues Organs; Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.; Authors - Hu Ping P