Anticorps Polyclonal de lapin anti-SLC22A2

SLC22A2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13594-1-AP

Synonymes

hOCT2, OCT2, Organic cation transporter 2, SLC22A2

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Galerie de données de validation

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  WB analysis using 13594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13594-1-AP (SLC22A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13594-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13594-1-AP (SLC22A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, tissu rénal de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13594-1-AP cible SLC22A2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC22A2 Protéine recombinante Ag4500
• Nom complet: solute carrier family 22 (organic cation transporter), member 2
• Masse moléculaire calculée: 555 aa, 63 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC039899
• Symbole du gène: SLC22A2
• Identification du gène (NCBI): 6582
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC22A2, also named as OCT2, belongs to the major facilitator (TC 2.A.1) superfamily and Organic cation transporter (TC 2.A.1.19) family. The SLC22A2 gene encodes for the organic cation transporter 2 (OCT2) protein which acts as a renal uptake transporter. Human OCT2 (hOCT2) is expressed on the basolateral side of the proximal tubule cells and plays a crucial role in the disposition and renal clearance of cationic drugs and endogenous compounds (PMID: 35143948). SLC22A2 has 3 isoforms with the molecular mass of 27, 54 and 63 kDa. Sometimes higher molecular weight around 70 kDa can also be observed, which may be a modified variant of SLC22A2.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Organic cation transporter 2 activation enhances sensitivity to oxaliplatin in human pancreatic ductal adenocarcinoma; Authors - Ching-Feng Chiu
human,mouse	WB	J Pharm Anal; Discovering metabolic vulnerability using spatially resolved metabolomics for antitumor small molecule-drug conjugates development as a precise cancer therapy strategy; Authors - Xiangyi Wang