Anticorps Polyclonal de lapin anti-SLC22A18

• Phare
• Validé par KD/KO

SLC22A18 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11646-2-AP

Synonymes

BWR1A, BWSCR1A, HET, IMPT1, ITM, ORCTL 2, ORCTL2, p45 BWR1A, SLC22A18, SLC22A1L, TSSC5

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Galerie de données de validation

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  WB analysis of mouse brain using 11646-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11646-2-AP (SLC22A18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11646-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11646-2-AP (SLC22A18 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11646-2-AP

  IP result of anti-SLC22A18 (IP:11646-2-AP, 4ug; Detection:11646-2-AP 1:600) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules COLO 320
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

11646-2-AP cible SLC22A18 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC22A18 Protéine recombinante Ag2245
• Nom complet: solute carrier family 22, member 18
• Masse moléculaire calculée: 408 aa, 43 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC015571
• Symbole du gène: SLC22A18
• Identification du gène (NCBI): 5002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC22A18 is a member of the family of polyspecific organic-cation transporters that localize at the apical membrane surface of renal proximal tubules. SLC22A18 is expressed at high levels in the kidney, liver, colon and in fetal renal proximal tubules and present at lower levels in heart, brain and lung. Defects in SLC22A18 are associated with breast and lung cancer and are the cause of rhabdomyosarcoma type 1, a malignant tumor derived from striated muscle. More recently, SLC22A18 has been shown to functions as a tumor suppressor in glioma and represents a candidate biomarker for long-term survival in this disease.

Publications

Species	Application	Title
human	WB	Int J Biol Macromol; microRNA-137 functions as a tumor suppressor in human non-small cell lung cancer by targeting SLC22A18.; Authors - Bicheng Zhang ; KD Validated
		Hum Mol Genet; Parent-of-origin tumorigenesis is mediated by an essential imprinted modifier in SDHD-linked paragangliomas: SLC22A18 and CDKN1C are candidate tumor modifiers.; Authors - Attje S Hoekstra
human	IHC	Cancer Epidemiol; Low expression of SLC22A18 predicts poor survival outcome in patients with breast cancer after surgery.; Authors - He Hongyu H