Anticorps Polyclonal de lapin anti-URAT1

• Phare
• Validé par KD/KO

URAT1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA, IF

Conjugaison

Non conjugué

Publications

73

N° de cat : 14937-1-AP

Synonymes

OAT4L, OATL4, Renal specific transporter, RST, SLC22A12, URAT1, Urate anion exchanger 1

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Galerie de données de validation

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  WB analysis using 14937-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 14937-1-AP

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, rein de rat, tissu rénal de porc

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 67 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Informations sur le produit

14937-1-AP cible URAT1 dans les applications de WB, IHC, ELISA, IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: URAT1 Protéine recombinante Ag6770
• Nom complet: solute carrier family 22 (organic anion/urate transporter), member 12
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 65-70 kDa, 40 kDa
• Numéro d’acquisition GenBank: BC053348
• Symbole du gène: URAT1
• Identification du gène (NCBI): 116085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo
mouse	WB	Mol Metab; URAT1-selective inhibition ameliorates insulin resistance by attenuating diet-induced hepatic steatosis and brown adipose tissue whitening in mice.; Authors - Yoshiro Tanaka
mouse	IHC	Food Funct; Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice; Authors - Arshad Mehmood
mouse	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang
rat	WB	Food Funct; Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota; Authors - Nanhai Zhang
mouse	WB	Nutrients; Chinese Sumac (Rhus chinensis Mill.) Fruits Prevent Hyperuricemia and Uric Acid Nephropathy in Mice Fed a High-Purine Yeast Diet; Authors - Nan Ma