Anticorps Polyclonal de lapin anti-SLC16A12

SLC16A12 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20553-1-AP

Synonymes

CJMG, MCT 12, MCT12, Monocarboxylate transporter 12, SLC16A12

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Galerie de données de validation

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  WB analysis of mouse kidney using 20553-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20553-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu rénal de souris, tissu rénal humain, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

20553-1-AP cible SLC16A12 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 16, member 12 (monocarboxylic acid transporter 12)
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53-56 kDa
• Numéro d’acquisition GenBank: NM_213606
• Symbole du gène: SLC16A12
• Identification du gène (NCBI): 387700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC16A12, also named as MCT12 and CJMG, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. It is a proton-linked monocarboxylate transporter. SLC16A12 catalyzes the rapid transport across the plasma membrane of many monocarboxylates. The antibody is specific to SLC16A12.

Publications

Species	Application	Title
Xenopus	WB	Biochem Biophys Res Commun; Molecular characterization of the orphan transporter SLC16A9, an extracellular pH- and Na+-sensitive creatine transporter.; Authors - Yuya Futagi
human	IHC	Medicine (Baltimore); Decreased expression of SLC16A12 mRNA predicts poor prognosis of patients with clear cell renal cell carcinoma.; Authors - Jie Mei