Anticorps Polyclonal de lapin anti-KCC2/SLC12A5-Specific

KCC2/SLC12A5-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 19565-1-AP

Synonymes

hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5-Specific, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

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Galerie de données de validation

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  WB analysis using 19565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19565-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 19565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody (19565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 19565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody (19565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

19565-1-AP cible KCC2/SLC12A5-Specific dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 12 (potassium-chloride transporter), member 5
• Masse moléculaire calculée: 126 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: NM_020708
• Symbole du gène: KCC2
• Identification du gène (NCBI): 57468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)

Publications

Species	Application	Title
human,mouse	WB,IF	Neurobiol Dis; HIV and opiates dysregulate K+- Cl- cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice.; Authors - Aaron J Barbour
mouse	IF	iScience; Time-restricted feeding entrains long-term behavioral changes through the IGF2-KCC2 pathway.; Authors - Qiaocheng Zhai
mouse	WB，IF	ASN Neuro; Restoration of KCC2 Membrane Localization in Striatal Dopamine D2 Receptor-Expressing Medium Spiny Neurons Rescues Locomotor Deficits in HIV Tat-Transgenic Mice.; Authors - Aaron J Barbour
rat	WB	Brain Behav; Involvement of the BDNF-TrkB-KCC2 pathway in neuropathic pain after brachial plexus avulsion.; Authors - Shuo Zhao
mouse	IHC	Front Mol Neurosci; Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion; Authors - Haijun Hu
mouse	WB	Ecotoxicol Environ Saf; 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway; Authors - Yuchen Qin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-04-2024)

KCC2 (in green) worked in my IF in human FPPE brain sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human Brain Cortex

KCC2/SLC12A5-Specific Antibody Immunofluorescence validation (1:50 dilution) in Human Brain Cortex (Cat no:19565-1-AP)