Anticorps Polyclonal de lapin anti-NKCC1,SLC12A2

• Phare
• Validé par KD/KO

NKCC1,SLC12A2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 13884-1-AP

Synonymes

Basolateral Na K Cl symporter, BSC, BSC2, NKCC1, SLC12A2

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Galerie de données de validation

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  WB analysis using 13884-1-AP

  Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with 13884-1-AP (NKCC1,SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de souris incubé à 37 °C
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

13884-1-AP cible NKCC1,SLC12A2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKCC1,SLC12A2 Protéine recombinante Ag4991
• Nom complet: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC033003
• Symbole du gène: NKCC1/SLC12A2
• Identification du gène (NCBI): 6558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

Publications

Species	Application	Title
mouse	WB,IHC	Science; A causative link between inner ear defects and long-term striatal dysfunction.; Authors - Michelle W Antoine
mouse	IHC	PLoS Biol; Early uneven ear input induces long-lasting differences in left-right motor function.; Authors - Michelle W Antoine
mouse	IHC	PLoS Biol; The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.; Authors - Krisztina Tóth
mouse	WB,IHC	J Neurosci; The severity of vestibular dysfunction in deafness as a determinant of comorbid hyperactivity or anxiety.; Authors - Michelle W Antoine
mouse	IF	Front Mol Neurosci; Unilateral optogenetic kindling of hippocampus leads to more severe impairments of the inhibitory signaling in the contralateral hippocampus; Authors - Fabio Cesar Tescarollo
human	WB	Sci Rep; Comprehensive analyses of solute carrier family members identify SLC12A2 as a novel therapy target for colorectal cancer; Authors - Dan-Yang Chen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Valentina (Verified Customer) (02-01-2023)

Multiple bands were detectable around 100 - 150 kDa, with different protocols and gels. The antibody is definitely working even if not optimally, probably necessary to try with a higher dilution and a lower amount of protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Choroid plexus homogenates