Anticorps Polyclonal de lapin anti-DMT1

• Phare
• Validé par KD/KO

DMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

84

N° de cat : 20507-1-AP

Synonymes

SLC11A2, Solute carrier family 11 member 2, OK/SW-cl.20, Natural resistance-associated macrophage protein 2, DMT-1

---

Galerie de données de validation

• 

  WB analysis using 20507-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 20507-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20507-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 20507-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20507-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse brain using 20507-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DMT1 antibody (20507-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 20507-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using DMT1 antibody (20507-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 20507-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DMT1 antibody (20507-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules Caco-2, cellules COLO 320, cellules Neuro-2a, cellules SH-SY5Y, tissu rénal de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'intestin grêle de souris, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 78 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Informations sur le produit

20507-1-AP cible DMT1 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DMT1 Protéine recombinante Ag14314
• Nom complet: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• Masse moléculaire calculée: 568 aa, 62 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC002592
• Symbole du gène: DMT1
• Identification du gène (NCBI): 4891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that play a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including the brain, kidney, testis, duodenum, and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Publications

Species	Application	Title
human	WB	Blood; Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.; Authors - Floriane Petit
human	IHC	Acta Neuropathol; Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.; Authors - Till S Zimmer
mouse	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
mouse	IF	Nat Commun; Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.; Authors - Hirofumi Yamaguchi
rat	WB	Transl Res; Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.; Authors - Guang Yang
mouse	WB	Redox Biol; Apolipoprotein E deficiency induces a progressive increase in tissue iron contents with age in mice.; Authors - Juan Ma